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Abstract:
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Halobacteria, members of the domain Archaea that live under extremely halophilic conditions,
are often considered as dependable source for deriving novel enzymes, novel genes, bioactive
compounds and other industrially important molecules. Protein antibiotics have potential for
application as preserving agents in food industry, leather industry and in control of infectious bacteria.
Halocins are proteinaceous antibiotics synthesized and released into the environment by
extreme halophiles, a universal characteristic of halophilic bacteria. Herein, we report the production
of halocin (SH10) by an extremely halophilic archeon Natrinema sp. BTSH10 isolated from salt
pan of Kanyakumari, Tamilnadu, India and optimization of medium for enhanced production of
halocin. It was found that the optimal conditions for maximal halocin production were 42 C,
pH 8.0, and 104 h of incubation at 200 rpm with 2% (V/V) inoculum concentration in Zobell’s medium
containing 3 M NaCl, Galactose, beef extract, and calcium chloride as additional supplements.
Results indicated scope for fermentation production of halocin for probable applications using halophilic
archeon Natrinema sp. BTSH10 |