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Abstract:
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Strain improvement is one of the major objectives for maximizing the
microbial production of industrially significant primary and secondary metabolites.
This goal can be achieved by judicious tuning of the organisms by monitoring its
growth parameters and optimizing adequate supply of micro and macro nutrients,
inducers, pH, temperature and other factors which control fermentation. Though C.
rugosa has been under extensive studies for lipases, maximum world production is
only 36 units. In fact, in India, enhanced production conditions for lipases have not
yet been initiated. C. rugosa has been cultivated in diverse environments like
liquid, semi-solid, solid—state and immobilized conditions, though major emphasis is
on SmF or suspension culture. Hence the present investigations mainly focused on
increasing the yield by adjusting the physico-chemical growth parameters and to
characterize the lipase isoforms secreted by C. rugosa in the culture medium.
Maximum possible improved methods were investigated to achieve these objectives.
Within this under-optimised background, enhancement of lipase production
and its characterization were investigated, employing modified liquid, semi-solid,
solid—state and immobilized fermentation strategies |