Ani Das, V; Paulose,C S(Department of Biotechnology, Faculty of Science, August , 2000)
[+]
[-]
Abstract:
In the present study, the changes in the brain EPI (Epinephrine), adrenergic receptors and the receptor gene expression were investigated during pancreatic regeneration and insulin secretion. The changes in the pancreatic islet EPI and adrenergic receptors were also studied in the pancreatectomised rats. The regulatory function of EPI in association with Epidermal growth factor (EGF) and glucose were investigated in rat islet cultures. In vitro studies were carried out using antagonists for adrenergic receptor subtypes to see their involvement in the islet DNA synthesis. The mechanism by which the peripheral EPI regulate insulin secretion was also investigated by studying the nuclear binding proteins in the pancreatic islets during pancreatic regeneration and diabetes. The study reveals that EPI can regulate the pancreatic islet cell proliferation by controlling the insulin synthesis and secretion. The brain adrenergic receptor gene expression and functional correlation regulate the pancreatic adrenergic receptors. The functional balance of α and β-adrenergic receptors controls the insulin secretion and pancreatic β-cell proliferation, which will have immense clinical significance in the treatment of Diabetes mellitus.
Remya, Robinson; Dr. Paulose, C S(Cochin University of Science and Technology, April , 2007)
[+]
[-]
Abstract:
In the present study a detailed investigation on the alterations of dopamine
and its receptors in the brain regions of streptozotocin induced diabetic and insulin
induced hypoglycaemic rats were carried out. Glutamate receptor, NMDARI gene
expression in the hypoglycaemic and hyperglycaemic brain was also studied. EEG
recording in hypoglycaemic and hyperglycaemic will be carried out to measure brain
activity. in vitro studies on glucose uptake and insulin secretion, with and without
specific antagonists were carried out to confirm the specific receptor subtypes - DA
D1, DA D2 and NMDA involved in the functional regulation during hyperglycaemic
and hypoglycaemic brain damage. The molecular studies on the brain damage
through dopaminergic and glutamergic receptors will elucidate the therapeutic role in
the corrective measures of the damage to the brain during hypoglycaemia and
hyperglycaemia. This has importance in the management of diabetes and antidiabetic
treatment for better intellectual functioning of the individual.
Description:
Department of
Biotechnology, Cochin University of Science and Technology