Niladevi, K N; Dr. Prema, P(National Institute for Interdisciplinary Science and Technology, 2008)
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Abstract:
The present study has identified an actinomycete culture (S. psammoticus) which
was capable of producing all the three major ligninolytic enzymes. The study
revealed that least explored mangrove regions are potential sources for the isolation
of actinomycetes with novel characteristics. The laccase production by the strain in
SmF and SSF was found to be much higher than the reported values. The growth of
the organism was favoured by alkaline pH and salinity of the medium. The enzyme
also exhibited novel characteristics such as activity and stability at alkaline pH and
salt tolerance. These two characters are quite significant from the industrial point of
view making the enzyme an ideal candidate for industrial applications. Many of the
application studies to date are focused on enzymes from fungal sources. However,
the fungal laccases, which are mostly acidic in nature, could not be used
universally for all application purposes especially, for the treatment of
effluents from different industries, largely due to the alkaline nature of the
effluents. Under such situations the enzymes from organisms like S.
psammoticus with wide pH range could play a better role than the fungal
counterparts. In the present study, the ability of the isolated strain and
laccase in the degradation of dyes and phenolic compounds was successfully
proved. The reusability of the immobilized enzyme system made the entire
treatment process inexpensive. Thus it can be concluded from the present
study that the laccase from this organism could be hopefully employed for
the eco-friendly treatment of dye or phenol containing industrial effluents
from various sources.
Soorej, Basheer M; Dr.Chandrasekaran,M(Cochin University of Science and Technology, April , 2007)
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Abstract:
The present study indicate the scope for the
utilization of the marine fungus Aspergillus awamori Nagazawa BTMFW 032 for
extracellular lipase production employing submerged fermentation. To the best of
our knowledge this is the first report on lipase production by a marine fungus
employing statistical modeling towards industrial production. The characterization
of purified lipase produced by A. awamori showed stability in organic solvents,
oxidizing agent and reducing agents, I,3-regiospecificity and hydrolytic activity.
These properties make this lipase an ideal candidate for biocatalysis in organic
media for the production of novel compounds such as biodiesel and sugar fatty
esters. 91.4 % reduction in oil and grease content in ayurvedic oil by the treatment
of A. awamori lipase indicates that there is a scope for this enzyme in the treatment
of oil effluents and bioremediation. There is ample scope for further research on
the biochemistry of the enzyme, structure elucidation and enzyme engineering
towards a wide range of further applications, besides enriching scientific
knowledge on marine enzymes.
Description:
Department of Biotechnology,
Cochin University of Science and Technology
Jissa, Krishna G; Dr.Chandrasekaran,M(Cochin University of Science and Technology, January , 2008)
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Abstract:
The marine microorganisms are yet to be exploited as a source of natural
pigments for probable utilization in various industries. Hence, in this study focus
was made only on pigment producing marine bacteria for pigment production and
evaluation of the same for some application besides development of an ideal
bioprocess for subsequent indigenous production of the pigment using the same
organism towards ultimate industrial application.
Description:
Department of Biotechnology, Cochin
University of Science and Technology