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| Abstract: | Considering the potential of marine environment present study was designed for the screening and isolation of a potential salt tolerant. alkaline and thennotolerant lipase producing bacteria from the costal belts of South India and consequent development of ideal bioprocess for industrial production, purification characterisation and evaluation of the potential of the lipase enzyme for various industrial applications 1. Screening and isolation of a potential lipase producing bacteria. 2. Optimization of various physicochemical factors in Submerged fennentation for the production of alkaline lipase 3. Purification ofthe lipase enzyme 4. Characterisation of the enzyme 5. Evaluation of the enzyme for various industrial applications |
| Description: | Microbial Technology Laboratory Department of Biotechnology Cochin University of Science and Technology |
| URI: | http://dyuthi.cusat.ac.in/purl/3969 |
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| Dyuthi-T1793.pdf | (4.124Mb) |
| Abstract: | Cochin, commercial capital of Kerala, located on the west-coast of South India has a large number of chemical and sea food industries. Earlier studies in the past indicated that these industries contribute to heavy metal pollution, particularly mercury, copper, and cadmium, in Cochin backwater. Hence, in the present study, it was desired to isolate cadmium resistant bacteria from effluent discharged by chemical industry with a view to develop an ideal bioremediation process for safe discharge of industrial effluent in to the nearby aquatic environment. Effluent from three industries, located in the industrial belt of Cochin, were collected from the discharge point and cadmium resistant bacteria were screened using standard microbiological techniques |
| Description: | Department of Biotechnology, Cochin University of Science and Technology |
| URI: | http://dyuthi.cusat.ac.in/purl/3640 |
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| Dyuthi-T1619.pdf | (2.331Mb) |
| Abstract: | There exists a need for potential microorganism that could facilitate effective bioremediation of crude oil pollutants in the environment. Hence it was desired to isolate a potential bacterium from marine sediment, which often experiences oil pollution and develop a bioprocess for crude oil biodegradation. In the present study the sediment deposits in the beach of Munakkal, Trichur dist, Kerala, collected immediately after the major event Tsunami in 2004 was collected and analyzed by enrichment culture technique towards isolation of potential strains that could degrade crude oil and its fractions. From the results obtained it was found that the sediment deposits harbor several bacteria with potential for degrading hydrocarbons. However, among the strains obtained, isolate no. BTTS 10 showed capabilities for utilizing both alkanes and aromatic hydrocarbons and hence the same was selected for further studies. |
| Description: | Department of Biotechnology, Cochin University of Science and Technology |
| URI: | http://dyuthi.cusat.ac.in/purl/3546 |
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| Dyuthi-T1514.pdf | (3.904Mb) |
| Abstract: | Many of the existing methods for the treatment of rubber latex centrifugation eflluent are not only unsatisfactory in their efliciency to effect near perfect treatment in bringing down the COD to optimum level, but also time consuming and need a large landspace. As the rate of effluent generation is extremely high (20 litres for kilogram of rubber) there is a need for development of efficient system,capable of rapid reduction of COD and BOD. Though the organic load of the rubber efiluent is very high, it does not contain much processed chemicals and therefore it can be considered as a ‘biological eflluent’. Further, the ratio of the Chemical Oxygen Demand to Biological Oxygen Demand (COD/BOD) of this effluent remain almost as a constant value. According to Montgomery (1967), estimation of BOD is not ideally suited for studies on process design, treatability, control of treatment plants, setting standards for treated effluents and assessing the effect of polluting discharges on the oxygen resources of receiving waters. Hence in the present study COD was measured to determine the impact of treatment system on the effluent. In the present study, attempts were made to evaluate the efficiencies of certain methods such as packed bed reactor using immobilized microbial cells, rotating biological contactor (RBC) and activated sludge process, for rapid and efficient treatment of natural rubber latex centrifugation effluent. In addition, studies were also carn'ed out to develop a suitable bioprocess for the coagulation of skim latex, as an alternative to the presently used acid coagulation process towards reducing the pollution load, besides recovering quality rubber |
| Description: | Department of Biotechnology, Cochin University of Science and Technology |
| URI: | http://dyuthi.cusat.ac.in/purl/3967 |
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| Dyuthi-T1791.pdf | (3.742Mb) |
| Abstract: | In the light of the very huge demand for natural ephedrine and pseudoephidrine, a search for an angiosperm plant containing the alkaloid ephedrine was made and could locate Sida spp. of malvaceae family. Sida is a large genus of, herbs and shrubs distributed throughout the tropics. About a dozen species occur in India. The medicinally important species known are S.rhombrfolia S.cordata and S.spinosa (Anon, 1972). Among the various species, S.rh0mbIfolia is the most widely used one in the traditional system of medicine. An attempt was made in the present study to develop an ideal bioprocess for the in vitro production of ephedrine from the cell culture system of Sida rhombrfolia Linn. ssp. retusa. The callus and suspension culture were initiated and attempts were made to enhance the yield positively by employing various strategies like mutagenesis, immobilization and addition of precursors, elicitors and penneabilizing agents. |
| Description: | Department of Biotechnology Cochin University of Science and Technology |
| URI: | http://dyuthi.cusat.ac.in/purl/3970 |
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| Dyuthi-T1794.pdf | (5.148Mb) |
| Abstract: | Microbial enzymes are in great demand owing to their importance in several industries such as brewing, baking, leather, laundry detergent, dairy. starch processing and textiles besides pharmaceuticals. About 80% of the enzymes produced through fermentation and sold in the industrial scale are hydrolytic enzymes. Due to recognition of new and new applications, an intensive screening of different kinds of enzymes with novel properties, from various microorganisms, is being pursued all over the world. Bacillus sp are largely known to produce a-amylase, among the different groups of microoganisms, at industrial level. They are known to produce both saccharifying and liquefying a-amylases (Fukumoto 1963; walker and Campbell, 1967a). which are distinguishable by their mechanisms of starch degradation by the fact that the saccharifying asamylases produce an increase in reducing power about twice that of the liquefying enzyme (Fukumoto, 1963; Pazur and Okada, 1966). Under this circumstances, the present study was undertaken, with a View to utilise a fast growing B.coagu1ans isolated from soil, for production of thermostable and alkaline oz-amylase under different fermentation processes |
| Description: | Department of Biotechnology, Cochin University of Science And Technology |
| URI: | http://dyuthi.cusat.ac.in/purl/3734 |
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| Dyuthi-T1688.pdf | (7.304Mb) |
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